以下为句子列表：
英文: 7kb exon6 to exon7 DNA sequence which is separated by PCR as Short Arm(SA),were used to construct HPRT gene knock out vector.
中文: 根据已知大鼠HPRT基因的外显子序列,从大鼠HPRT基因BAC中用酶切和PCR方法分别分离得到用于构建基因敲除载体的3。
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英文: 8 PCR fragments corresponding to alleles of 8 SSR loci were cloned and sequenced from 8 common almonds.
中文: 共获得8个PCR片断,共计个等位变异片断。
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英文: (3) Universal DNA polymerase was used in PCR amplification instead of Pfu enzyme;
中文: (3)在PCR反应中用普通的DNA聚合酶代替高保真酶(Pfu);
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英文: 3. Combining the in silico cloning, RACE and RT-PCR method, the full-length cDNA sequence of PSMC1, PSMC4 and PSMC5 gene and the entire coding region of the PSMC2 and PSMD4 genes were cloned.
中文: 3．利用电脑克隆、RACE和RT-PCR的方法相结合，克隆了蛋白酶体激活因子PA700相关基因PSMC1、PSMC4和PSMC5共3个基因的全长cDNA序列，以及PSMC2和PSMD4基因的完整编码区(coding sequence，CDS)。 进行了蛋白质序列的推导和功能域的预测。
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英文: 3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
中文: 经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。
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