英文: The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight
was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein.
中文: 通过三批重复实验，确定了rIL－2工程菌高密度培养方案，平均菌密度A600nm值为58，菌干重为18．0g／L，rIL－2表达占菌体总蛋白的42．4％。 更详细...
英文: When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight
and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively.
中文: 在3.7 L台式搅拌发酵罐中,控制溶氧在30%以上,重组菌的对数生长中期流加碳源和氮源,最终菌体光密度A600达到52.4,菌体干重为26.2g/L,目的蛋白的表达量达到24.8%。 更详细...
英文: When the strain was cultivated in a 5 L fermenter,dry cell weight
increased to 8.06 g/L from 3.12 g/L. The high-cell density growth of engineering strain has no negative effect on the expression of target protein, and the production of rhAGN was 2.51 g/L.
中文: 利用此合成培养基进行5 L罐的发酵试验,菌体干重由摇瓶下的3.02 g/L提高到8.06 g/L,同时,试验表明,菌体浓度提高后,目的蛋白的表达未受影响,依然稳定在35%左右,rhAGN的表达量达到2.51g/L。 更详细...