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The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein.
中文意思:
通过三批重复实验,确定了rIL-2工程菌高密度培养方案,平均菌密度A600nm值为58,菌干重为18.0g/L,rIL-2表达占菌体总蛋白的42.4%。


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The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein. 通过三批重复实验,确定了rIL-2工程菌高密度培养方案,平均菌密度A600nm值为58,菌干重为18.0g/L,rIL-2表达占菌体总蛋白的42.4%。
The temperature for Streptomyces albulus to grow was 28 C,the yield of glucose to Streptomyces albulus was 0.429 g. cell/g. sub, the field of Streptomyces albulus was 10.07g/L after the batch fermentation, the field of 拢 -PL was only 0.79g/L. 28℃时对细胞生长非常有利,菌体得率Y_(x/s)达到0.429g.cell/g.sub,发酵结束时菌体量最高为10.07g/L,但ε-聚赖氨酸产量仅0.79/L。
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The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF). 方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。
 
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