|
| When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively. |
中文意思：
在3.7 L台式搅拌发酵罐中,控制溶氧在30%以上,重组菌的对数生长中期流加碳源和氮源,最终菌体光密度A600达到52.4,菌体干重为26.2g/L,目的蛋白的表达量达到24.8%。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
|
|
We identified 4 genes, theexpression of three (Beclin 1, RbAp48 and Pir51) were increased and one(aldolase b) was decreased in liver tumor tissues.
|
|
|
我们选择其中 4 个基因(Beclin1、RbAp48、Pir51 和 aldolase b)进行 Northern Blotting 及 RT-PCR 验证,结果显示这 4 个基因在肝癌与癌旁组织中差异率大于 50%。 |
|
We observed the effect of inopolysaccharide of Spirulina maxima(IPS)on the growth and apoptosis of SMMC7721,A549,HeLa,U251 tumor cell lines.
|
|
|
研究了极大螺旋藻(Spirulina maxima)胞内多糖对SMMC7721等肿瘤细胞系的生长及凋亡的影响。 |
|
We reviewed the advances of detective methods of new immunology and gene on gastric carcinoma metastasis, and discussed the combinative application of multi-markers detection.
|
|
|
综述了有关胃癌浸润转移的免疫分子和基因的研究现状 ,提出了新的免疫学诊断指标在预测肿瘤转移方面的应用 ,以及多项指标联合检测的可能性 |
|
We take the method of Proteinase K to extract RNA from the virus extraction and we find a single RNA with the length of 15,000 bp.
|
|
|
对病毒的精提纯液用蛋白酶K消化法提取RNA,电泳检测到一约15000bp的单链核酸。 |
|
When detecting foot-and-mouth disease virus VPl in distilled RNA, When the quantity of nucleic acid exceed or be equal to 0. 23ng/μl, RT-PCR can detect;
|
|
|
通过部标RT-PCR方法和nested PCR方法的敏感性比较研究,部标RT-PCR方法能检测出样品中病毒核酸量大于或等于0.23ng/μl的被检材料; |
|
When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively.
|
|
|
在3.7 L台式搅拌发酵罐中,控制溶氧在30%以上,重组菌的对数生长中期流加碳源和氮源,最终菌体光密度A600达到52.4,菌体干重为26.2g/L,目的蛋白的表达量达到24.8%。 |
|
When the body is unable to endure the antigens of islets, both MHC-Ⅱand its disposel antigens of β cells would activate Th1 subgroup ofhelper T lymphocytes (Th), and restrain Th2 subgroup, which results inthe unbalance of cytokines.
|
|
|
当机体对胰岛多种抗原成分失去耐受后,主要组织相容性复合物(MHC)-Ⅱ类分子与其处理的β细胞抗原共同激活辅助性 T 淋巴细胞(Th)中的 Th1 亚群,抑制 Th2 亚群,造成 Th1/Th2 型细胞因子的不平衡; |
|
When the bone marrow is preserved in liquid nitrogen, the viability and function of the cells can be sustained much longer. The recoverable rate of the nuclear cells, exclusion of trypon blue, DNA and CFU-C arc 76%, 89%, 77% and 79% respectively.
|
|
|
骨髓贮存于液氮中,其细胞的活力和功能得以较长保存,有核细胞、锥蓝拒染率、脱氧核糖核酸(DNA)和CFU—C的回收率分别为76%、89%、77%和79%. |
|
When the direction of modification is parallel to the basic vector of Si (111) 7×7 surfaces, grooves formed by such a process have atomically straight edges and lateral features as small as one 7×7 unit cell wide.
|
|
|
当这种表面改形的取向与Si(111)7×7表面晶格基矢方向平行时,形成的沟槽将具有愿子级平直的边缘,横向宽度仅为一个7×7晶胞。 |
|
When the strain was cultivated in a 5 L fermenter,dry cell weight increased to 8.06 g/L from 3.12 g/L. The high-cell density growth of engineering strain has no negative effect on the expression of target protein, and the production of rhAGN was 2.51 g/L.
|
|
|
利用此合成培养基进行5 L罐的发酵试验,菌体干重由摇瓶下的3.02 g/L提高到8.06 g/L,同时,试验表明,菌体浓度提高后,目的蛋白的表达未受影响,依然稳定在35%左右,rhAGN的表达量达到2.51g/L。 |
|
When treated with 100mg/L、250mg/L or 500mg/L Ag+, the cell wall was loosely, uneven, and the widened space between cell wall and membrane was seen clearly.
|
|
|
Ag+含量100mg/L、250mg/L、500mg/L处理组作用后，可见到金葡菌细胞壁松散，胞壁与胞膜分离等不同程度的破坏，菌体肿胀，胞浆电子密度降低，直至菌体破裂。 |
|
|
|
