以下为句子列表：
英文: The best condition selected in this study for the protoplast preparation is that: 15 hr for cell age . 0. 01 M for pretreatment concentration of β-mercaptoethanol and about 60 min for enzymolysis time.
中文: 经实验确认,原生质体制备最优条件为:取菌龄为15hr的菌体,以0.01mol/L的β—巯基乙醇预处理,蜗牛酶温浴时间为60min.
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