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restriction enzyme

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限制; 限制性内切


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英文: Abstract:The polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by PCR,restriction enzyme analysis and electrophoresis methods.The results showed the distribution of genetypes i

中文: 摘要本文采用PCR、限制性酶切和电泳分型等方法,分别对90例原发性高血压患者和109例正常人血管紧张素原基因多态位点AGT174进行了检测,结果表明,高血压组中三种基因型的分布与对照组显著不同,提高该位点变异与原发性高血压的发生相关。        更详细...
英文: A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid (DNA) by capillary electrophoresis with laser induced fluorescence detection (CE-LIFD) was developed.

中文: 建立了毛细管电泳分离 激光诱导荧光检测(CE LIFD)分析分枝杆菌脱氧核糖核酸(DNA)限制性内切酶谱的新方法。        更详细...
英文: Approximately 3.5 kb hTERT gene was cut by EcoRⅠ and BamHⅠ from the retroviral vector pBABE-hTERT-puro and then inserted to the corresponding restriction enzyme sites of pBABE-puro-lox to make a new vector phTERTPlox.

中文: 用EcoRⅠ和BamHⅠ酶切下逆转录病毒载体pBABE-hTERT-puro上约3.5kbhTERT基因片段,插入pBABE-puro-lox载体的相应酶切位点之间,重组的新载体称为phTERTPlox;        更详细...
英文: In 1987, the notion of a splicing system was introduced by Tom Head in [3] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of DNA molecules.

中文: 1987年,Tom Head发表了一篇论文,引入拼接系统(splicing system)的概念作为限制酶与DNA(脱氧核糖核酸)作用、进行DNA重组的数学模型。        更详细...
英文: [Methods] Linearized pBABE-puro was cut by restriction enzyme NheⅠand ligated with loxP double strands to construct a vector pBABE-puro-lox.

中文: 方法用NheⅠ酶切线性化pBABE-puro产生两个相同的黏端,与loxP双链混合连接构建成载体pBABE-puro-lox;        更详细...
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