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[Methods] Linearized pBABE-puro was cut by restriction enzyme NheⅠand ligated with loxP double strands to construct a vector pBABE-puro-lox.
中文意思:
方法用NheⅠ酶切线性化pBABE-puro产生两个相同的黏端,与loxP双链混合连接构建成载体pBABE-puro-lox;


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[Methods] Linearized pBABE-puro was cut by restriction enzyme NheⅠand ligated with loxP double strands to construct a vector pBABE-puro-lox. 方法用NheⅠ酶切线性化pBABE-puro产生两个相同的黏端,与loxP双链混合连接构建成载体pBABE-puro-lox;
[Methods] The serum levels of IL-15 were measured by a double-antibody sandwich ELISA in 27 severe hepatitis B patients, 20 chronic hepatitis B patients and 20 healthy subjects, parameters of liver function, PT and HBV DNA were detected in the same time. 方法采用双抗体夹心酶联免疫吸附试验检测27例重型乙型肝炎、20例慢性乙型肝炎患者及20例健康体检者血清IL-15水平,并同期检测患者肝功能、PT和HBV DNA等指标。
[Objection] To study the level changing of T cell subgroup Th1/Th2 cytokine and the relation of cytokine changing with liver function ALT and serum HBVDNA tite in the chronic hepatitis B patients accepted INFα-2a therapy. 目的探讨慢性乙肝患者接受干扰素α-2a(INFα-2a)抗病毒治疗后血清T淋巴细胞亚群Th1/Th2细胞因子水平的变化及与肝功能ALT恢复、血清HBVDNA含量变化的关系。
[Objective] To analyze cause of the liver damage after renal transplantation, and to summarize the therapy of the liver damage in hepatic virus. 目的通过对肾移植术后患者肝功损害的原因分析,总结乙肝病毒对肾移植受体肝脏的损害及治疗措施。
[Objective] To explore the relationship between polymorphisms of DNA repair gene XRCC1 and susceptibility to radiation injury. [目的]研究DNA修复基因XRCC1多态性与辐射损伤易感性的关系。
[Objective] To investigate CD1 and cytokines expression in patients with systemic lupus erythematosus (SLE). 目的研究系统性红斑狼疮(SLE)患者外周血CD1与细胞因子的表达关系。
 
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