以下为句子列表：
英文: MethodsNucleotides within glycosylation motif were changed to a human mutant CD9 gene(from K-H to K-K) by overlap extension PCR. The mutant CD9 gene was inserted into a eukaryotic-expressing vector pALTER to form a recombinant plasmid.
中文: 方法以正常的CD9基因全长cDNA序列为模板,应用重叠延伸PCR法产生突变使CD9糖基化基序K-H突变为K-K,产生突变CD9基因,将其克隆于pALTER质粒中,构建出重组真核表达载体。
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英文: the glycosylation of the lactase protein is . % gained by sulfuric acid -phenol method.
中文: 硫酸-苯酚法测得其糖基化程度为. %。
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英文: Progress in Methods of Glycosylation Analysis and its Related Application in Proteomics
中文: 蛋白质糖基化分析方法及其在蛋白质组学中的应用
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英文: Protein Glycosylation Engineering
中文: 蛋白质糖基化工程
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