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| MethodsNucleotides within glycosylation motif were changed to a human mutant CD9 gene(from K-H to K-K) by overlap extension PCR. The mutant CD9 gene was inserted into a eukaryotic-expressing vector pALTER to form a recombinant plasmid. |
中文意思：
方法以正常的CD9基因全长cDNA序列为模板,应用重叠延伸PCR法产生突变使CD9糖基化基序K-H突变为K-K,产生突变CD9基因,将其克隆于pALTER质粒中,构建出重组真核表达载体。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
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Methods:Using Langendorff perfusion technique,the ischemia reperfusion injury model was established by ceasing perfusion for minutes,and reperfusion for minutes.
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方法：采用Langendorf灌流技术，以停灌注分钟、再灌注分钟造成缺血再灌注损伤模型，观察缺血预处理对缺血再灌注损伤心肌的保护作用，及洛沙坦（losartan）对这一作用的影响。 |
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Methods:Using physiological saline to fill into the domestic rabbit's stomach (0 ml/single), 0 minutes later, Lingui Ganzao Decoction ig (weight∶0 ml/kg), recording urinate volume with BL-0 organism function experiment system.
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方法 :家兔用生理盐水灌胃 ( 0 ml/只 ) ,0 min后以苓桂甘枣汤水煎液灌胃 (0 ml/kg体重 ) ,BL - 0生物机能实验系统记录排尿量。 |
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Methods:Wavelength of Zinc Hallow Cathode Modulation( .9 nm) was used to determine zinc in water and food and then the wavelength was changed into copper wavelength( .8 nm) to determine copper in water and food without any change of determing condition.
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方法:采用锌空心阴极灯波长( .9 nm)测定水与食品中锌,再把波长改变成铜的波长( .8 nm)其它测定条件不变,测定水与食品中铜。 |
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Methods:applied the methods of Aschoff' entrainment,we obversed the phase response curve of electro-acupuncturing "Baihui"(Du0)、"Changqiang"(Du),8-OH-DPAT,and electro-acupuncture associated with 8-OH-DPAT on hamster spontaneous behavior circadian rhythm.
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方法:运用Aschoff导引方法,对比观察电针“百会”、“长强”、8-OH-DPAT、电针+8-OH-DPAT对金黄地鼠自发活动近似昼夜节律的影响及其相位反应曲线特点。 |
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MethodsA retrospective analysis was made in cases of TAD from September 998 to December 00 Of them, 87 received inguinal oblique incisions, hypogastric retroperitoneal incisio ns and retroperitoneal incisions prolonged by inguinal oblique incisions.
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方法 回顾性分析 998年 9月至 0 0 年 月间胸主动脉夹层腔内隔绝术 例 ,其中选择腹股沟斜切口 87例 ,下腹部腹膜外切口 例 ,由腹股沟斜切口延长为下腹部斜切口 例。 |
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MethodsNucleotides within glycosylation motif were changed to a human mutant CD9 gene(from K-H to K-K) by overlap extension PCR. The mutant CD9 gene was inserted into a eukaryotic-expressing vector pALTER to form a recombinant plasmid.
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方法以正常的CD9基因全长cDNA序列为模板,应用重叠延伸PCR法产生突变使CD9糖基化基序K-H突变为K-K,产生突变CD9基因,将其克隆于pALTER质粒中,构建出重组真核表达载体。 |
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MethodsReaction of L-α-Val with ZnAc_·H_O in methanol yields the clubbed crystal, which is identified as Zn(Val)_·H_O by chemical and elemental analyses.
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果 化学分析与元素分析结果表明,其可能组成为Zn(Val)·HO。 用X 射线衍射法测定了它的晶体结构,属正交晶系,P空间群,晶胞参数为:a=0 7 (0)nm; |
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Method：Nineteen embalmed pelves and articulated proximal / of the femurswere harvested for this study.
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髋臼后壁骨折测量完整髋臼、后壁 / 、/ 、 / 骨折时髋臼与股骨头之间的接触特性---负重、负重面积、平均应力、峰值应力和应力的分布情况。 |
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Methof:The mm defects of rat ischium nerve were treated with allografted nerves kept at - 0℃ for 0 days.
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方法：用— 0℃保存0天的同种异体神经桥接大鼠坐骨神经mm缺损； |
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Methord:We extend sclera valve area( mm× mm),imprest infiltration. insure ideal postoperation eye pressure and prevent scar.
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方法 :扩大巩膜瓣设计面积 (mm× mm) ,加强滤过 ,确保理想的术后眼压 ,防止瘢痕形成。 |
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Methylbenzoethyl)sydnone and -( -hydroxyl- -phenylpropyl)sydnone undergo Mannich reaction with aromatic amines and give the corresponding Mannich bases.
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(甲基苯甲酰基乙基 )斯德酮和 ( 羟基 苯基丙基 )斯德酮可以与芳香胺发生Mannich反应 ,得到斯德酮与芳香胺的Mannich碱 |
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