以下为句子列表：
英文: Construction of eukaryotic expression vector containing human GM-CSF andB7. fusion gene from a single cistron.
中文: 预防接种瘤苗三次后接受野生型 EL- 细胞(×0 个)攻击,EL-/GM-CSF组(每组均为 8 只)有 只小鼠、EL-/B7. 组有 只小鼠、EL-/GM-B7. 组有 只小鼠长期无瘤生存。
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英文: Aim:To construct coexpression vector of pETDuet-aroB-qutB and transform it into E. coli,in which two key enzymes of dehydroquinate synthase(DHQase) and quinate dehydrogenase(QDHase) in quinic acid biosynthesis were highly coexpressed.
中文: 目的:构建共表达载体pETDuet-aroB-qutB,在大肠杆菌中同时表达奎尼酸合成途径中的关键酶脱氢奎尼酸合成酶(DHQase)和奎尼酸脱氢酶(QDHase)。
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英文: After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).
中文: 将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。
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英文: Cell Cycle Related Protein Overexpression in Squamous Carcinoma of the Tongue and Effects of Sense and Antisense Cyclin A Expression Vector on Tca8113 Cell Line
中文: 细胞周期相关蛋白在舌癌中的表达及正反义cyclin A基因转染对舌癌细胞Tca8113的生物学效应
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英文: Construction and expression of prokaryotic expression vector of Cp The recombinant plasmids pET-28a-15,pET-28a-15/60 and pET-28a-23 were constructed by cloning CP15,CP15/60 and CP23 genes into prokaryotic expression vector pET-28a(+) and expressed in E.
中文: 原核表达载体的构建及表达 将3个基因片段克隆到pET－28of＋）载体上构建成3个原核表达载体：pET－28a－15、pET－28al5／60和pET－28a－23，经酶切鉴定后在宿主菌BLZI（DE3）中表达。
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