以下为句子列表：
英文: DNA polymerase α-DNA primase complex from K cells, a human chronic myelogenous leukemia cell strain, was purified by sequential chromatography of phosphocellulose, DEAE-cellulose, single-strand DNA cellulose and AMP-sepharose columns.
中文: 用磷酸纤维素、DEAE纤维素、单链DNA纤维素和AMP-Sepharcse的顺序柱层析法从人慢性骨髓细胞性白血病K细胞中纯化了DNA聚酶α与DNA引物酶的复合物。
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英文: The inclusion body washed with % deoxycholic acid sodium salt and dissolved with 0.% N-lauroyl sarcosine sodium was recovered by the way of dialysis,then the protein was purified with Glutathione Sepharose TM~(B) and its purity was above 8%.
中文: 以包涵体形式存在的重组蛋白以%的脱氧胆酸钠洗涤、0.%的N-十二烷基肌氨酸钠溶解、透析复性、Glutathione Sepharose TMB纯化后,纯度达8%以上。
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英文: The process was as follows: 0.g of CNBr-activated sepharose B was swelled by mmol/L of HCl,and mixed with AChE solution with the activity of 0U,then,the mixture was shaked for 8 hours at ℃.
中文: 制备方法为:0.g CNBr活化的Sepharose B凝胶用mmol/L的HCl充分溶胀后,与活力为0U的AChE溶液混合,于℃下0r/min振荡8h。
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英文: The specific activity of GST from midgut reached \{ 888.\} (nmol/(min·mg)) by GSH-Sepharose B column chromatography and the purification factor increased to 07. 8-fold.
中文: 用GSH_Sepharose B纯化中肠GST,GST比活力达到.888 nmol (min·mg), 纯化倍数达到07. 8。
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英文: The studied on coupling DSA with CNBr-activated Sepharose 4B under different pH condition
中文: 两种pH值条件下CNBr活化Sepharose 4B偶联DSA方法的比较研究
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