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DNA polymerase α-DNA primase complex from K cells, a human chronic myelogenous leukemia cell strain, was purified by sequential chromatography of phosphocellulose, DEAE-cellulose, single-strand DNA cellulose and AMP-sepharose columns.
中文意思:
用磷酸纤维素、DEAE纤维素、单链DNA纤维素和AMP-Sepharcse的顺序柱层析法从人慢性骨髓细胞性白血病K细胞中纯化了DNA聚酶α与DNA引物酶的复合物。


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DNA Sequence Analysis for the Promoter of Pyruvate Oxidase Gene from Strptococcus Oralis 口腔链球菌丙酮酸氧化酶基因调节区的DNA序列分析
DNA and RNA in . g/(kg·d) hongjingtian group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. 红景天.g/(kg·d)剂量组的DNA和RNA分布均匀,荧光反射边界清晰,反应强度明显增强,与假手术组及川芎嗪80mg/(kg·d)对照组表达分布基本相同。
DNA and RNA in sham-operation group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. 假手术组DNA和RNA分布均匀,荧光反射边界清晰,反应强度较强;
DNA homology analysis showed that strains in Phenon and of numerical taxonomy were A. DNA-DNA杂交结果表明,数值分类群和群的菌株分别为A.
DNA polymerase a is required for the initiation of DNA replication and the priming of Okazaki fragments during elongation. 如具有引物酶活性的聚合酶α的作用是启动DNA复制并合成RNA-DNA引物;
DNA polymerase α-DNA primase complex from K cells, a human chronic myelogenous leukemia cell strain, was purified by sequential chromatography of phosphocellulose, DEAE-cellulose, single-strand DNA cellulose and AMP-sepharose columns. 用磷酸纤维素、DEAE纤维素、单链DNA纤维素和AMP-Sepharcse的顺序柱层析法从人慢性骨髓细胞性白血病K细胞中纯化了DNA聚酶α与DNA引物酶的复合物。
DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras)and of IL-, IL- receptor, T-cell receptor β-chain and transferrin receptor were dotted onto nitrocellulose filters. mRNA,以之为模板通过逆转录法得到以α-~ P标记的第一链cDNA,再以cDNA为探针同abl,myc,myb,fos,ras五种癌基因及TcR-β,IL-,IL-R,TfR四种与细胞生长增殖密切相关的基因片段进行斑点杂交。
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