以下为句子列表：
英文: AIM:To investigate the effect of hypoxia-inducible transcription factor-1α(HIF-1α)on proliferation of and the VEGF expression in SW480 cells,so as to explore the possible mechanism of HIF-1α regulation on cell proliferation.
中文: 目的:研究转录因子-缺氧诱导因子-1α(HIF-1α)在缺氧条件下对人结肠癌SW480细胞增殖的作用,以及HIF-1α对血管内皮生长因子(VEGF)表达的影响,研究HIF-1α调节SW480细胞增殖的机制。
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英文: Immortalization of embryonic fibroblasts in heat shock transcription factor 1 knockout mouse;
中文: HSF1基因敲除小鼠胚胎成纤维细胞的永生化
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英文: 3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
中文: 经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。
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英文: Before treating, IgE in serum of the patients were higher than those of the healthy controls (P<0. 05) , and the Th2 Types Cytokines in serum(IL-4.10) , the expression of Transcription factor GATA-3mRNA in PBMC were higher too (P<0. 05) .
中文: AD患者的治疗前血清IgE水平显著高于正常对照组(P<0.01),Th2型细胞因子(IL-4、IL-10)较正常对照组明显升高(P<0.05),Th1型细胞因子(IL-12)较正常对照组明显降低(P<0.05),外周血PBMC中GATA-3mRNA水平显著高于正常对照组(P<0.05)。
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英文: 【Methods】Reverse transcription polymerase chain reaction(RT-PCR) was used to detect TLR4 mRNA expression,and enzyme-linked immunosorbent assay(ELISA) was used to examine IL-8 secretion.
中文: 【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达; 酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。
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