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【Methods】Reverse transcription polymerase chain reaction(RT-PCR) was used to detect TLR4 mRNA expression,and enzyme-linked immunosorbent assay(ELISA) was used to examine IL-8 secretion.
中文意思:
【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达; 酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。


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③ liver function of the two groups one month after operation:The abnormal high glutamic-pyruvic transaminase in the Mycophenolate group was significantly lower as compared with Mizoribine group(13.3%,34.6%,P < 0.05). ③两组患者术后1个月的肝功能比较:吗替麦考酚酯组患者的谷丙转氨酶升高发生率显著低于咪唑立宾组(13.3%,34.6%,P<0.05);
③The detection rate of HBV preS1 antigen was high in liver fuction(ALT 40 U/L)of chronic active hepatitis group. ③慢性活动性肝炎肝功能指标ALT>40 u/l组其HBV PreS1抗原检出率高。
③vWF expression of cardiac muscle in each group: 2 weeks after operation, the SABC showed markedly positive expressions of vWF in MSCs transplantation group, and obvious decrease was observed in model control group and cell medium group. ③各组大鼠心肌组织血管性假血友病因子表达情况:链霉亲和素免疫组化法显示,造模后2周干细胞移植组心肌细胞血管性假血友病因子呈强阳性表达,模型对照组和细胞培养基组血管性假血友病因子表达减弱。
④The levels of E-selectin, intercellular adhesion molecule-1(ICAM -1)and vascular cell adhesion molecule-1 (VCAM-1) in supernatant of HUVECs was detected by enzyme-linked immunosorbent assay (ELISA). ④吸取各组上清液,按酶联免疫吸附试剂盒说明操作分别测定各孔可溶性E-选择素、细胞间黏附分子1、血管细胞黏附分子1的含量。
⑤The c-kit positive cells were about 55.3% polygon cells in cell membrane and cytoplasm,whereas fibroblast-like cells with smaller bodies were negative for c-kit. ⑤体积较大的多角形细胞为c-kit阳性,体积较小的成纤维样细胞为阴性细胞,阳性颗粒主要分布在细胞膜和细胞质,细胞核未见表达,阳性细胞比率为55.3%。
【Methods】Reverse transcription polymerase chain reaction(RT-PCR) was used to detect TLR4 mRNA expression,and enzyme-linked immunosorbent assay(ELISA) was used to examine IL-8 secretion. 【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达; 酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。
㎡bserve the effect of GAPSGF on the serum protein components by SDS-PAGE. ③通过SDS-PAGE,观察GAPSGF对HepA瘤血清蛋白组分的影响;
Results:The difference of the ratio of liver function recuperated and the time of HBV-DNA turned negative in two groups had no significant meaning(P>0.05). 结果:两组肝功能复常率、HBV-DNA转阴时间比较,差异无显著性意义(P>0.05);
Effect of Cigarette Smoke on Maturation of DNA in Sperm Nucleus and Function of Sperm Membrane in Rats 香烟烟雾对大鼠精子核DNA成熟度和精子膜功能的影响
Isolation and Cultivation of Murine Hematopoietic Stem Cells and Expression of hFIX Mediated by Recombinant Lentiviral Vectors in vitro 小鼠造血干细胞的分离、培养及重组慢病毒载体介导的离体hFⅨ基因表达
A study on the interaction between dye and bovine serum albumin 染料分子与牛血清蛋白相互作用的研究(英文)
 
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