以下为句子列表：
英文: In recent years, a number of rational and combinatorial protein engineering projects have focused on extending and tailoring CAL-B's catalytic and physical properties.
中文: 近年来，一系列组合蛋白质工程研究和扩展了南极假丝酵母脂肪酶的催化和物理特性。
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英文: Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases.
中文: 研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。
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