以下为句子列表：
英文: Methods: Serum levels of TNF-α were measu red by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 15 patients with psoriasis arthropathica before and after treatment with MTX.
中文: 方法:应用双抗体夹心酶联免疫吸附法(ELISA),检测15例关节病型银屑病患者经MTX治疗前后外周血清中TNF-α的水平。
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