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Methods: Serum levels of TNF-α were measu red by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 15 patients with psoriasis arthropathica before and after treatment with MTX.
中文意思:
方法:应用双抗体夹心酶联免疫吸附法(ELISA),检测15例关节病型银屑病患者经MTX治疗前后外周血清中TNF-α的水平。


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Methods sICAM-1 and L-selectin were detected by enzyme-linked immunosorbent assay(ELISA) and IL-2,IL-6,IL-8 and IL-10 by radioimmunoassay(RIA) in the serum samples from 24 SLE cases and 20 healthy volunteers. 方法采自24例SLE患者的血清标本分别用酶联免疫吸附试验(ELISA)检测sICAM-1和L-选择素; 用放射免疫检测法(RIA)检测IL-2、IL-6、IL-8和IL-10;
Methods to achieve web page multimedia-aided teaching of pharmacology by Dreamweaver 用Dreamweaver实现药理学网页多媒体教学的几种方法
Methods: A set of reverse- nested- polymerase chain react ion (RT- nested -PCR ) was conducted by using primers designed according to the 5'-noncoding sequence in patients with HGV infection, to detect the RNA of HGV (HGV - RNA). 方法:根据HGV感染者5’-非编码区的核苷酸序列设计引物,应用逆转录套式聚合酶链反应(RT-nested-PCR)对庚型肝炎病毒核糖核酸(HGV-RNA)进行检测。
Methods: Cholesterol(50.00 mg/L) was used on human umbilical endothelial cell line,ECV304,48 h later,cell genomic DNA methylation level was detected by HPLC. 方法:体外培养人脐静脉血管内皮细胞ECV304,以50.00 mg/L的胆固醇作用于ECV304 48 h,提取细胞基因组DNA,用高效液相色谱法(HPLC)检测基因组DNA甲基化水平。
Methods: Classified 39 liver recipients according to treatment protocol (basic treatment, added HGF treatment, adbed RNA treatment) as control group, HGF group and RNA group. 方法:将病人分为Control组、HGF组、RNA组,分别采用基础治疗、基础治疗+肝细胞生长因子、基础治疗+核糖核酸3种治疗方法。
Methods: Serum levels of TNF-α were measu red by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 15 patients with psoriasis arthropathica before and after treatment with MTX. 方法:应用双抗体夹心酶联免疫吸附法(ELISA),检测15例关节病型银屑病患者经MTX治疗前后外周血清中TNF-α的水平。
Methods: The experiment was performed in 6 adult rabbits with the method of retrograde axonal transportation of horseradish peroxidase (HRP), by which HRP was smeared on the proximal cut end of the left ilioinguinal nerves. 方法:成年家兔6只,采用HRP逆行追踪方法,在左侧髂腹股沟神经近侧断端涂抹HRP。
Methods: The levels of IL-12 were measured by enzyme linked immunosorbent assay(ELISA) in 64 patients with viral hepatitis and 20 healthy adults subject as controls. 方法:双抗体夹心酶联免疫吸附法(ELISA)检测64例病毒性肝炎患者及20例正常对照血清IL-12水平。
Methods: The liver function and renal function in 268 cases of hemorrhagic fever with renal syndrome were tested and compared with 30 cases of healthy control group. 方法:对268例HFRS患者的肝功能和肾功能进行检测,并与30例健康对照组进行比较。
Methods: The quantitative study was made in 47 samples of different ovarian tumor cell DNA content. 方法 :对 47例不同性质的卵巢肿瘤细胞脱氧核糖核酸 (deoxyribonucleicacid ,DNA)含量进行定量研究。
Methods: The specific IgM antibodies of EV,HSV-I,HSV-II,EBV,ADV and FluV were determined by enzyme-linked immunosorbent assay(ELISA) in the cerebrospinal fluid(CSF) in 92 viral encephalitis and meningitis cases. 方法:应用酶联免疫吸附(ELISA)方法对92例临床诊断为病毒性脑炎、脑膜炎患儿的脑脊液进行肠道病毒(EV)、单纯疱疹病毒(HSV)I型和II型、EB病毒(EBV)、腺病毒(ADV)及流感病毒(FLuV)特异性IgM抗体检测。
 
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