以下为句子列表:
英文: Achievement of research and development of Hydroxyproline (Hyp) is reviewed with 37 cited references, including four parts, i.e., unbalanced Hyp level and diseases, detecting methods and their application, manufacture techniques and prospects.
中文: 摘要从羟脯氨酸平衡失调与疾病、检测方法与应用、生产工艺、展望等4个方面介绍了羟脯氨酸的研究与开发。
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英文: (3) Hyp was purified after cell collection, suspension, ammonium sulfate precipitation and Ni-NTA column chromatography.
中文: 3、离心发酵液以收集菌体,用缓冲液悬浮菌体,经过超声破碎、硫酸铵分级沉淀和Ni-NTA亲和层析,得到了电泳纯的Hyp。
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