以下为句子列表：
英文: Dominating methods in wheat gene cloning include homology-based cloning, map-based cloning, suppression subtractive hybridization, DDRT-PCR etc.
中文: 目前，小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
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英文: Detection of cyclin B level by RT-PCR and its significance for primary hepato cellular carcinoma
中文: 半定量RT-PCR检测原发性肝癌cyclinB的表达和意义
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英文: 3. Combining the in silico cloning, RACE and RT-PCR method, the full-length cDNA sequence of PSMC1, PSMC4 and PSMC5 gene and the entire coding region of the PSMC2 and PSMD4 genes were cloned.
中文: 3．利用电脑克隆、RACE和RT-PCR的方法相结合，克隆了蛋白酶体激活因子PA700相关基因PSMC1、PSMC4和PSMC5共3个基因的全长cDNA序列，以及PSMC2和PSMD4基因的完整编码区(coding sequence，CDS)。 进行了蛋白质序列的推导和功能域的预测。
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英文: 3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
中文: 经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。
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英文: Changes of the expression of protein and mRNA of Bcl-2 gene were examined by S-P immunohistochemical staining and RT-PCR analysis respectively.
中文: 以S-P免疫组化及逆转录聚合酶链反应(RT-PCR)法分别检测Bcl-2基因蛋白与mRNA的表达变化。
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