以下为句子列表：
英文: A 40-base polymorphic repeat sequence located in the 3'-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
中文: 将位于多巴胺运输器基因3'端未转译区段的40-硷基多形性重复序列予以纯化、经聚合酶链锁反应放大。
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英文: A brief introduction to the X-ray crystallographic studies on RNA polymerase Ⅱ complexes and the enzymatic mechanisms revealed by the crystal structures.
中文: 摘要用X射线晶体学方法测定的一系列RNA聚合酶Ⅱ复合物结构揭示了真核转录的分子机制。
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英文: (3) Universal DNA polymerase was used in PCR amplification instead of Pfu enzyme;
中文: (3)在PCR反应中用普通的DNA聚合酶代替高保真酶(Pfu);
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英文: A Preliminary Study on DNA Polymerase of Cell L_(7712)
中文: L_(7712)细胞DNA聚合酶的初步研究
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英文: 【Methods】Reverse transcription polymerase chain reaction(RT-PCR) was used to detect TLR4 mRNA expression,and enzyme-linked immunosorbent assay(ELISA) was used to examine IL-8 secretion.
中文: 【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达; 酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。
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