以下为句子列表：
英文: Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ.
中文: 方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。
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英文: The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T-A clone technique and was digested with restriction enzyme of Kpn I and BamH I.
中文: 通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。
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