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| The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T-A clone technique and was digested with restriction enzyme of Kpn I and BamH I. |
中文意思：
通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。 |
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The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T-A clone technique and was digested with restriction enzyme of Kpn I and BamH I.
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通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。 |
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