英文: Abstract: The diferrences in cell growth, composition and levels of anthocyanins and catechins, activity of phenylalanine
ammonia-lyase and dynamics of production between anthocyanin accumulating and non-accumulating subclones of tea cultured cells were e
中文: 文摘:从细胞生长、色素和儿茶素次生产物组成与含量、苯丙氨酸解氨酶活性以及产物合成动态分析几方面，研究比较了积累色素与不积累色素的两类茶树培养细胞系之间的差异。 更详细...
英文: It was found that the levels of catechins and proanthocyanins in those subclones accumulating anthocyanins were also raised, and were independent of the activity of phenylalanine
中文: 发现积累色素的细胞系中，儿茶素与原花色素的形成能力也得到了大幅度提高，且与苯丙氨酸解氨酶活性无关。 更详细...
英文: A Zorbax C8 column was used with the mobile phase of methanol-chiralsolution(containing 8mmol/L L-phenylalanine
and mmol/L cupric sulfate, adjusted to pH . by NaOH solution) (∶8)at the detection wavelength of nm. The detection limit was ng.
中文: 采用Zorbax C8柱,甲醇-手性溶液(8mmol/L L-苯丙氨酸和mmol/L硫酸铜混合溶液,用NaOH溶液调至pH .)(∶8)为流动相,检测波长为 nm,光学异构体可被良好分离,检测限ng。 更详细...
英文: Chorismate mutase (CM ), prephenate dehydratase (PD ), and phenylalanine
aminotransferase (PAT) are the three key enzymes in the fermentation of phenylalanine
. In E. colt,the first two are encoded by a single gene pheA,while the third by tyrB gene.
中文: 为探讨用基因工程的手段改良苯丙氨酸的发酵菌株，采用聚合酶链反应（PCR）的方法，从大肠杆菌总DNA中克隆得到了编码苯丙氨酸合成途中的两个关键酶基因－－即分枝酸变位酶（CM）／预苯酸脱水酶（PD）基因pheA与苯丙氨酸转氨酶（PAT）基因tyrB，在大肠杆菌中进行了这两个基因的单个和串联表达。 更详细...
英文: In phosphate buffer (pH8.0) of 0 mg/ml wet cells of the engineering L.lactis and 0 mmol/L phenylalanine
were incubated at 7℃ for 8 h, and the conversion rate reached %.
中文: 以0mmol/L的苯丙氨酸作为底物,在pH8.0的磷酸盐缓冲液中,于 7℃反应8h,其转化率可达%。 更详细...