以下为句子列表：
英文: Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene.
中文: 插入突变是一种通过外源DNA整合的方式来获得突变体，并克隆得到对应突变基因的方法。
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英文: Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases.
中文: 研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。
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英文: Methods:The objective gene to be mutated was cloned into pAlter Ex plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.
中文: 方法 :将待突变的目的基因克隆到 pALTER Ex载体上 ,采用寡核苷酸介导的定点突变进行突变。
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英文: An Enzymatic Method for Random- (Site-Specific)Mutagenesis of Ginseng peptide Gene in vitro
中文: 人参多肽基因的酶法体外随机——定位诱变
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英文: EFFECT OF MUTAGENESIS AT Ser 177 RESIDURE IN PENICILLIN G ACYLASE ON ACTIVITY OF THE ENZYME
中文: 青霉素G酰化酶α亚基Ser177的突变对酶活性的影响
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