以下为句子列表：
英文: By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein, to determine the secondary structure of a protein, and to explore the unfold-refold mechanism of a protein.
中文: 运用圆二色谱和荧光技术分析预测蛋白质二级结构，研究蛋白质的稳定性与蛋白质的变性复性动力学机制。
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英文: Refold along the crease, enclosing mixture, and make a double 1/2-inch fold along the 3 open sides.
中文: 依照折痕，再次折叠，包住食物，在其他三个开放面上也重复折叠两次，每次1/2英寸。
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英文: This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli, and summarize the most efficient ways to refold recombinant proteins.
中文: 摘要描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性，综述了国内外变性、复性的有效方法。
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英文: We have used two different operating schemes, fed-batch and step-addition, independently and collaboratively to refold denatured lysozymes.
中文: 本研究中，我们利用馈料式与间歇式不同的复性方法，以独立地或结合地方式进行溶菌酶之复性。
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