"In Utero Electroporation"即为子宫内电穿孔技术,这是一种利用电穿孔技术将基因引入子宫器官内的一种技术,主要用于研究基因或蛋白质的功能.该技术在国际上应用很多,可以在pubmed上查到相应文献.

细胞电穿孔的研究始于70年代。所谓电穿孔(Electroporation)即是在外加短时强电脉冲时,在细胞膜双脂层上形成瞬时微孔,使细胞膜的通透性增强,离子,亲水分子,病毒颗粒,DNA,蛋白质以及染料颗粒等正常情况下不能通过细胞膜的分子得以进出细胞的一个生物物理过程。目前,电穿孔技术已广泛用于生物技术,基因工程,临床医学等许多领域。利用电穿孔可实现基因转染,细胞融合,以及将外源蛋白质分子插入细胞膜或导入细胞,激活细胞膜的传输因子,提高酶的活性,并可提高药物进入细胞的能力,增强癌症的化疗效果。
参考 *低强度瞬态电磁脉冲对细胞膜的电穿孔效应及机理研究*

参考操作步骤实例:
In utero electroporation
7. Inject 1-3 μl of the DNA solution into the ventricle using a mouth-controlled pipette system with the micropipette.
？We can inject the solution approximately into an aimed site, because the surface of the telencephalon is visible through the uterin wall, by illumination of a fiber-optics light source.
8. Hold a DNA-injected embryo in parallel along its antero-posterior axis through the uterus with forceps-type electrodes, and deliver electric pulses to the embryo.
？The electric pulses are generated using ElectroSqurePorator T820 (BTX, San Diego CA) and each pulse is for duration of 50 ms.
？Fill a gap between the electrodes with warm saline*, before electric pulses.
9. Put back the uterus carefully into the abdominal cavity.
10. Fill the cavity with warm saline*.
11. Sew up the abdominal wall and skin with the surgical needle and thread.
12. Warm the mouse on a hot plate at 38°C, until the mouse recovers from the anesthetic.