以下为句子列表：
英文: Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases.
中文: 研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。
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英文: Methods:The objective gene to be mutated was cloned into pAlter Ex plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.
中文: 方法 :将待突变的目的基因克隆到 pALTER Ex载体上 ,采用寡核苷酸介导的定点突变进行突变。
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英文: Gene Cloning,Site-directed Mutagenesis and Prokaryotic Expression of Porcine Sep15
中文: 硒蛋白Sep15基因克隆、定点突变及其原核表达
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英文: Characterization and Site-directed Mutagenesis of Xylanase 1YNA from Thermomyces lanuginosus DSM10635
中文: 嗜热真菌木聚糖酶1YNA的表达和定点突变
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英文: Studies on the Site-directed Mutagenesis of the Disulfide Linkage Cys129-Cys232 of Trypsin
中文: 胰蛋白酶分子中二硫键Cys129－Cys232的定位改造研究
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