以下为句子列表：
英文: Methods: Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture.
中文: 方法：采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。
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英文: The best digestion time determined by trypsin digestion method for collecting neural stem cells derived from spinal cord
中文: 胰酶消化法收集脊髓神经干细胞最佳消化时间的选择
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