以下为句子列表：
英文: Abstract: Three theory problems about technology of leather making are discussed.The first is the difference between surface charge and isoelectric point (PI).The second is about the origin of the calculation formula of basicity in making Cr3+ liquor.The
中文: 文摘:本文讨论了皮革制造中的三个理论问题，一是表面电荷与电点(PI)之间差别，二是三价铬鞣液配制中公式的简单来源，三是生皮在碱液中膨胀的机理。
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英文: Methods: In the present experiment, acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels were used and the amino acid composition and isoelectric point of porcine platelet-derived growth factor were studied.
中文: 方法：用酸水解及聚丙烯酰胺凝胶等电聚焦的方法测定猪血小板衍生生长因子的氨基酸含量、组成成分和等电点。
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英文: Objective: To determine the amino acid composition and isoelectric point of porcine platelet-derived growth factor.
中文: 摘要目的：测定猪血小板衍生生长因子的氨基酸组成和等电点。
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英文: Strain E could secrete alkaline protease, whose molecular weight was 9.0 kDa, isoelectric point was 9. and Km value was .× 0~(- ) g/mL.
中文: 菌株E所分泌的碱性蛋白酶的分子量为9.0kDa，等电点为9. ，米氏常数Km为.×0~(- )g／mL。
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英文: Calculation on Isoelectric Point and Net Charge of Peptide
中文: 多肽净电荷数及等电点的计算
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