以下为句子列表：
英文: Method:the following five mutations were detected with specific PCR of allelic gene for two couples and their high risky fetus:CD-(-CTTT),IVSⅡ(C→T),CD7(A→T),TATA box nt-8(A→G)and CD7-7(+A).
中文: 方法:应用等位基因特异性聚合酶链反应(PCR)技术检测两对夫妇及其高风险胎儿的种β-地中海贫血突变类型:CD-(-CTTT)缺失突变、IVSII(C→T)突变、CD7(A→T)无义突变、TATA盒nt-8(A→G)突变和CD7-7(+A)移码突变。
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