以下为句子列表：
英文: Establishing a human osteosarcoma cell line of stably-transfected vitamin D receptor antisense cDNA;
中文: 稳定转染维生素D受体反义cDNA的人骨肉瘤细胞系的建立
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英文: Compared with MHCC97-H cells, KAI protein staining in the MHCC97-H-S cells transfected with sense KAI expression plasmid was obviously enhanced(Integra Oculus Dehter ,IOD 0.7±.099 vs .7±.9,P<0.0).
中文: 免疫细胞化学SP法检测显示,转入正义KAI基因后的肝癌细胞KAI蛋白染色加深,(细胞积分光密度 integra oculus dehter,IOD0.7 ± .099 vs .7±.9,P<0.0);
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英文: Effects of Ly9A transfected lymphocytes on experimental graft versus host disease post allogeneic bone marrow transplantation
中文: 转移杀伤细胞抑制性受体Ly9A的淋巴细胞对异基因骨髓移植后移植物抗宿主病的作用
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英文: HMGB and EGFP were subcloned into hytohemagglutinin (HA) tagged vector pcDNA HA by two steps, the construct of which was then transfected into 9 A cells and observed with fluorescence microscope.
中文: 通过两步亚克隆的方法,将HMGB和EGFP的编码序列以融合表达形式克隆到带有血凝素(hytohemagglutinin,HA)标记的载体pcDNA HA上,随后转染9 A细胞,在荧光显微镜下观察结果。
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英文: METHODS: Two recombinant pALTER plasmids containing two hmCD9 cDNAs were transfected into CHO cells respectively with pcDNA by lipofectamine.
中文: 方法: 分别构建两种含有hmCD9全长cDNA序列的重组pALTER质粒, 运用脂质体介导法, 与pcDNA 质粒共转染CHO细胞, 以G8筛选阳性克隆(编号为hmCD9- - CHO及hmCD9 -- CHO)。
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