以下为句子列表：
英文: Abstract：Based on present plant gene engineering techniques, some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations
中文: 摘要本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。
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英文: Based on present plant gene engineering techniques, some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations or co-tr
中文: 本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。
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英文: Aim:To construct coexpression vector of pETDuet-aroB-qutB and transform it into E. coli,in which two key enzymes of dehydroquinate synthase(DHQase) and quinate dehydrogenase(QDHase) in quinic acid biosynthesis were highly coexpressed.
中文: 目的:构建共表达载体pETDuet-aroB-qutB,在大肠杆菌中同时表达奎尼酸合成途径中的关键酶脱氢奎尼酸合成酶(DHQase)和奎尼酸脱氢酶(QDHase)。
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英文: After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).
中文: 将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。
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英文: By expression vector pHHSM002, tpsl gene also expressed efficiently in E. coli BL21(DE3)PlysS ,the target protein amount to 17.8% in cell.
中文: 以pHHSM002为表达载体，tps1基因在大肠杆菌BL21中也得到了高效表达，表达的目的蛋白占菌体总蛋白的17.8％。
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