以下为句子列表：
英文: The mRNA and protein expression of DDC were examined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting methods, respectively.
中文: 方法:体外合成多巴脱羧酶的小干扰RNA(siRNA-DDC),转染人胃癌细胞系BGC823,逆转录酶链式反应(RT-PCR)和免疫印迹法(Western blot)方法检测DDC基因和蛋白表达的变化,体外侵袭实验检测转染后细胞侵袭能力的变化。
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英文: Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out.
中文: 方法应用RT-PCR、免疫印迹和免疫组织化学染色方法,检测出生后15d、35d、70d小鼠精囊腺、前列腺AQP1 mRNA、蛋白表达水平及细胞定位。
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英文: Method Enzyme-linked Immuno-sorbent Assay (ELISA) and western blotting were used to detect the antibody produced by the 95 patients with SARS and test the specificity of virus spike protein antigen.
中文: 方法运用酶联免疫法(ELISA)和蛋白质印迹法(WesternBlotting)检测95例SARS患者体内抗体的产生情况和验证病毒Spike蛋白抗原的特异性。
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英文: Protein tyrosine phosphorylation in platelet s was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
中文: 用蛋白质免疫印迹法 (Westernblotting)测定血小板内蛋白酪氨酸磷酸化的表达。
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英文: Methods Levels of Lp(a) were measured with enzyme-linked immunosorbent assay(ELISA) and genetic phenotypes of Apo(a) were mapped using SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting analysis.
中文: 方法应用酶联免疫吸附试验测量血清脂蛋白(a)浓度应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western blotting技术显示载脂蛋白遗传表型。
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