以下为句子列表：
英文: Conclusion The full length of marmot Interleukin-0 cDNA was successfully cloned and the recombinant plasmid pMD8-T-cmIL-0 was constructed.
中文: 论成功克隆旱獭IL-0分子,并构建重组质粒pMD8-T-cmIL-0。
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英文: Results The ×myc tagged recombinant plasmid pcDNA .0- ×myc /hIL-7RLM-L was generated successfully and its expression can be detected by Western blot in eukaryotic cells.
中文: 果 成功地构建了带有 ×myc标签的 pcDNA .0 ×myc /hIL 7RLM L重组质粒 ,Westernblot检测到该质粒可在真核细胞中表达。
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英文: Conclusion: Using recombinant plasmid pET28a(+)-GNLY/BL21(DE3)pLysS expression systems, the GNLY protein with biologic activity could be produced, and the strategy is available to prepare human GNLY in large quantity by gene engineering technique.
中文: 结论:用含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS表达系统,可得到具有生物活性的重组颗粒溶素,为大批量生产提供了条件。
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英文: Objective: To establish the high-expression recombinant plasmid pGEX-NT3 in order to obtain lots of human neurotropin 3 protein with biological activity.
中文: 目的：通过构建高效表达载体pGEX-NT3的重组质粒并在JM109宿主菌中进行表达，希望获得具有生物活性的人NT3蛋白，为大量获得重组人NT3纯品打下一定的实验基础。
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