以下为句子列表：
英文: Under this condition,the optimal pH for immobilized enzyme was 4.5,the optimal temperature was 50℃,and Km value was 14.29 g/L. Compared with the free enzyme ,its stability and repetition were considerably improved.
中文: 壳聚糖酶在经固定化后,最适温度为50℃,最适pH为4.5,并表现出比游离酶更高的热稳定性,固定化酶的米氏常数Km值为14.29 g/L;
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