英文: Chorismate mutase (CM ), prephenate de
hydratase (PD ), and phenylalanine aminotransferase (PAT) are the three key enzymes in the fermentation of phenylalanine. In E. colt,the first two are encoded by a single gene pheA,while the third by tyrB gene.
中文: 为探讨用基因工程的手段改良苯丙氨酸的发酵菌株,采用聚合酶链反应(PCR)的方法,从大肠杆菌总DNA中克隆得到了编码苯丙氨酸合成途中的两个关键酶基因--即分枝酸变位酶(CM)/预苯酸脱水酶(PD)基因pheA与苯丙氨酸转氨酶(PAT)基因tyrB,在大肠杆菌中进行了这两个基因的单个和串联表达。
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英文: dehydroquinate de
hydratase was a(α2/β2)3 peptide,of which a pair of anti-parallel β-strands existed in the N-terminus and a loop in the C-terminus;
中文: 脱氢奎尼酸酶结构域为(α2β2)3多肽,在N-端有一对反平行的β链,在C-端有loop环;
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