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METHODS: The experiment was performed in the Pharmacology Laboratory of Jinzhou Medical College from October 2005 to May 2006. Fifty SD rats were randomly divided into five groups: sham, model, ZG groups of 167, 250 and 357 mg/kg.
中文意思:
方法:实验于2005-10/2006-05在锦州医学院药理学实验室进行,50只SD大鼠随机分为5组,即假手术组,缺血再灌注模型组,葡萄糖酸锌167,250,357mg/kg剂量组。


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METHOD OF MEASURE GLYCOSYLATED SERUM PROTEIN WITH α-D-GLUCOSE PENTAACETATE(α-D-GPA) AS A STANDARD REFERENCE MATERIAL AND STUDY OF REACTIVE MECHANISM 以α-D-GPA为标准参照物测定糖化血清蛋白的方法及其反应机理初探
METHOD:after cultured 72 hour in vitro,cytokines (IL-2、IL-4、IL-10、IFN-γ、TNF-α) in supernatant secreted by PBMCs were detected by ELISA. HCV RNA in serum were assayed by fluorescent quantitation PCR. 方法:体外培养慢性HCV感染患者PBMCs72小时后,ELISA检测培养上清细胞因子(IL-2、IL-4、IL-10、IFN-γ、TNF-α)含量,荧光定量PCR检测患者血清HCV RNA含量。
METHODS 110 batches of FFP, human immunoglobulin and human serum albumin were detected for anti-HCV, HCV RNA, anti-HIV (1+2) 、HIV-1RNA by ELISA, western blot (WB) and PCR. 方法用ELISA、WB和PCR对110批FFP、人血丙种球蛋白、人血白蛋白检测了抗HCV、HCVRNA、抗HIV(1+2)、HIV-1RNA。
METHODS the pharmacokinetics of EPC and HEPC sterically stabilized liposomes (EPC-SSL, and HEPC-SSL) were studied by HPLC. 方法用高效液相色谱法研究EPC和HEPC长循环阿霉素脂质体在大鼠体内的药物动力学。
METHODS: Reverse transcription polymerase chain reaction (RT-PCR) technique was used to detect the expression of β-G mRNA in 10 cases of normal liver tissue and 38 cases of different differentiation tissues of hepatocellular carcinoma. 方法采用逆转录-聚合酶链反应(RT-PCR)方法,对10例正常肝脏及38例不同分化程度肝癌组织中β-G mRNA的表达进行对比研究。
METHODS: The experiment was performed in the Pharmacology Laboratory of Jinzhou Medical College from October 2005 to May 2006. Fifty SD rats were randomly divided into five groups: sham, model, ZG groups of 167, 250 and 357 mg/kg. 方法:实验于2005-10/2006-05在锦州医学院药理学实验室进行,50只SD大鼠随机分为5组,即假手术组,缺血再灌注模型组,葡萄糖酸锌167,250,357mg/kg剂量组。
METHODS: The pcDNA3.1-FKBP12.6 plasmid, mingled with albumin-coated microbubbles agents, was transfected into H9c2 (2-1) cells by ultrasound-mediated destruction of microbubbles. The H9c2 (2-1) cell growth state was investigated by inverted microscope. 方法:将pcDNA3.1-FKBP12.6质粒与白蛋白包裹微泡造影剂混合,经超声转染H9c2(2-1)细胞后,通过倒置显微镜观察心肌细胞生长状况的变化;
METHODS: The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively. 方法:采用[~3H]TdR与[~3H]脯氨酸掺入分别测定脱氧核糖核酸和胶原合成。
METHODS: To observe the effect of propafenone, we gave various concentration gradient (1, 6, 10 μmol/L) on the APD of endocardium myocytes in ischemic state with whole cell patch-clamp through alterring frequency of stimulation. 方法采用全细胞膜片钳技术,观察在不同刺激频率(即基础循环周长,BCL=2000、1000、500、250ms)下,不同浓度(1、6、10μmol/L)的普罗帕酮对缺血心肌左右心室心内膜心肌细胞动作电位时程(APD)的影响。
METHODS: healthy malevolunteers (age 22.7 ±0.7 years old,rang 22-24 years old, weight 60.6 ±4.5kg)werestudied in order to understand the pharmacokinetics of GFA. 为研究单次静脉注射盐酸GFA人体内药代动力学。 方法:健康男性10名,年龄:22岁-24岁(平均22.7±0.7岁);
METHODS: ① The experiment was done in the laboratory of molecular immunology, Weifang Medical College from October 2002 to October 2003. Thirty healthy male Wistar rats aged 8 weeks old were selected. 方法:①实验于2002-10/2003-10在潍坊医学院分子免疫实验室完成。 选用30只8周龄健康雄性Wistar大鼠。
 
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