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The expressions of cytokines essential for the growth of hEG,namely basic fibroblast growth factor(bFGF) and leukemia inhibitory factor(LIF),were also examined by RT-PCR.
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应用RT-PCR检测hEG生长所需的重要细胞因子的表达,即碱性成纤维细胞生长因子(bFGF)和白血病抑制因子(LIF)。 |
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The extraction of nucleic acid is the foundation in molecular biological experiments, and RNA with high quality is the premise for cDNA library construction and genes expression.
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核酸提取是分子生物学实验的基础,提取高质量的核糖核酸(RNA)是构建cDNA文库和进行基因表达研究工作的前提。 |
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The feasibility to improving rooting ability and topical dominance with both rolB and pttGA20ox genes at the same time was proved by transforming mode plant tobacco.
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通过转化模式植物来验证同时转化rolB及pttGA20ox两个基因促进植物生根及顶端优势的可行性。 |
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The fingerprintings were more clearer when the genome DNA was 100ng/μl.
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白色念珠菌基因组DNA含量在100ng/μl时,得到的指纹图谱最为清晰; |
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The first group included 19 cases with fatty liver(38.78%); the second group included 29 cases without fatty liver(61.22%). Blood lipids in 2 groups were analyzed with biochemical method.
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所有患儿分为脂肪肝组19例(38.78%)及无脂肪肝组29例(61.22%),对2组血脂成分进行分析。 |
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The fluorescence spectra of 9-(3-aminoanilino)acridine(B2,m-APAA) with Deoxyribonucleic acid(DNA) has been studied, so a fluorescent method for the determination of DNA was established;
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对9-(3-氨基苯胺基)吖啶(B_2，简称m-APAA)与脱氧核糖核酸(DNA)作用的荧光光谱进行了研究，拟定了测定DNA的分析方法，探讨了反应机理，实验了最佳条件、干扰实验。 |
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The four enzyme s belonging to classⅠ,ADH,HK,PFK and PDC,all catalyze irreversible reaction s.
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属于第一大类的四种酶ADH、HK、PFK和PDC,均催化不可逆反应。 |
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The function of antisense PIP RNA on HBV PRE-dependent post-transcriptional regulation
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PIP反义核酸对乙肝病毒PRE转录后调节的影响 |
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The fusion protein was soluble and over 30% of the total protein in the supertanant after ultrasonication .
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表达的融合蛋白主要以可溶形式存在于宿主细胞内，超声破菌后，融合蛋白的含量占上清蛋白总量的30%以上。 |
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The gene chip used in this study contained 407 genes.
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本研究还针对407个基因(包括信号传导有关的基因及转录因子)制备了基因芯片。 |
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The genotypes of their serum HBV were detected by using PCR integrated with Tagman MGB probe technology, and their serum HBV markers, HBV DNA and liver functions were also examined.
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采用PCR结合Taqman MGB探针技术,对血清中HBV基因型进行分型检测,同时检测HBV血清标志物、HBV DNA、肝功能。 |
