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| METHODS: Reverse transcription polymerase chain reaction (RT-PCR) technique was used to detect the expression of β-G mRNA in 10 cases of normal liver tissue and 38 cases of different differentiation tissues of hepatocellular carcinoma. |
中文意思：
方法采用逆转录-聚合酶链反应(RT-PCR)方法,对10例正常肝脏及38例不同分化程度肝癌组织中β-G mRNA的表达进行对比研究。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
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METHOD Human umbilicus vein endothelial cell and lipopolysaccharide were used to establish apoptosis cell models,and flow cytometer and confocal microscope were used to observe the effect of the medicated serum on ICAM-1 expression and intracellular Ca2+.
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方法取人脐静脉内皮细胞(HUVEC),用脂多糖造成凋亡细胞模型,通过流式细胞仪、激光共聚焦显微镜观察丹参药物血清对ICAM-1表达及胞内Ca2+的影响。 |
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METHOD OF MEASURE GLYCOSYLATED SERUM PROTEIN WITH α-D-GLUCOSE PENTAACETATE(α-D-GPA) AS A STANDARD REFERENCE MATERIAL AND STUDY OF REACTIVE MECHANISM
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以α－D－GPA为标准参照物测定糖化血清蛋白的方法及其反应机理初探 |
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METHOD:after cultured 72 hour in vitro,cytokines (IL-2、IL-4、IL-10、IFN-γ、TNF-α) in supernatant secreted by PBMCs were detected by ELISA. HCV RNA in serum were assayed by fluorescent quantitation PCR.
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方法:体外培养慢性HCV感染患者PBMCs72小时后,ELISA检测培养上清细胞因子(IL-2、IL-4、IL-10、IFN-γ、TNF-α)含量,荧光定量PCR检测患者血清HCV RNA含量。 |
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METHODS 110 batches of FFP, human immunoglobulin and human serum albumin were detected for anti-HCV, HCV RNA, anti-HIV (1+2) 、HIV-1RNA by ELISA, western blot (WB) and PCR.
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方法用ELISA、WB和PCR对110批FFP、人血丙种球蛋白、人血白蛋白检测了抗HCV、HCVRNA、抗HIV（1＋2）、HIV－1RNA。 |
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METHODS the pharmacokinetics of EPC and HEPC sterically stabilized liposomes (EPC-SSL, and HEPC-SSL) were studied by HPLC.
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方法用高效液相色谱法研究EPC和HEPC长循环阿霉素脂质体在大鼠体内的药物动力学。 |
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METHODS: Reverse transcription polymerase chain reaction (RT-PCR) technique was used to detect the expression of β-G mRNA in 10 cases of normal liver tissue and 38 cases of different differentiation tissues of hepatocellular carcinoma.
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方法采用逆转录-聚合酶链反应(RT-PCR)方法,对10例正常肝脏及38例不同分化程度肝癌组织中β-G mRNA的表达进行对比研究。 |
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METHODS: The experiment was performed in the Pharmacology Laboratory of Jinzhou Medical College from October 2005 to May 2006. Fifty SD rats were randomly divided into five groups: sham, model, ZG groups of 167, 250 and 357 mg/kg.
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方法:实验于2005-10/2006-05在锦州医学院药理学实验室进行,50只SD大鼠随机分为5组,即假手术组,缺血再灌注模型组,葡萄糖酸锌167,250,357mg/kg剂量组。 |
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METHODS: The pcDNA3.1-FKBP12.6 plasmid, mingled with albumin-coated microbubbles agents, was transfected into H9c2 (2-1) cells by ultrasound-mediated destruction of microbubbles. The H9c2 (2-1) cell growth state was investigated by inverted microscope.
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方法:将pcDNA3.1-FKBP12.6质粒与白蛋白包裹微泡造影剂混合,经超声转染H9c2(2-1)细胞后,通过倒置显微镜观察心肌细胞生长状况的变化; |
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METHODS: The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively.
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方法:采用[~3H]TdR与[~3H]脯氨酸掺入分别测定脱氧核糖核酸和胶原合成。 |
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METHODS: To observe the effect of propafenone, we gave various concentration gradient (1, 6, 10 μmol/L) on the APD of endocardium myocytes in ischemic state with whole cell patch-clamp through alterring frequency of stimulation.
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方法采用全细胞膜片钳技术,观察在不同刺激频率(即基础循环周长,BCL=2000、1000、500、250ms)下,不同浓度(1、6、10μmol/L)的普罗帕酮对缺血心肌左右心室心内膜心肌细胞动作电位时程(APD)的影响。 |
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METHODS: healthy malevolunteers (age 22.7 ±0.7 years old,rang 22-24 years old, weight 60.6 ±4.5kg)werestudied in order to understand the pharmacokinetics of GFA.
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为研究单次静脉注射盐酸GFA人体内药代动力学。 方法：健康男性10名，年龄：22岁－24岁（平均22．7±0．7岁）； |
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