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| Methods The human breast cancer tissues and relatively normal breast tissues were collected from surgical operation and the samples of 21 patients with breast cancer were analyzed by immunocytochemistry. |
中文意思：
方法收集21例手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法对不同乳腺组织标本进行检测。 |
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43 patients as the control group were given caryophyllin 4 mL i.
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方法将87例患者随机分成两组,治疗组44例,给予复方甘草酸苷注射液100mL+5%葡萄糖注射液250mL/d,静脉滴注,1次/d,1个月后改为口服复方甘草酸苷片50mg,3次/d,连用两月为1个疗程;对照组43例,给予肝炎灵4mL/d,肌肉注射,3个月为1个疗程。 |
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The effects of different kinds of Ca2+ channel antagonists on ICa were observed by 40 ms depolaring pulse applied from a holding potential of -70 mV in 10 mV steps up to ± 60 mV.
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方法利用全细胞膜片钳技术,在维持电压-70 mV的条件下,给予细胞40 ms,从-60mV到+60 mV的序列除极方波,阶跃10 mv,引导出钙通道电流(ICa),观察各种不同浓度PKA激动剂对大鼠嗜铬细胞ICa的影响。 |
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Objective To search a new method for screening high-risk group of cervical carcinoma.
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方法免疫组织化学技术检测13例慢性宫颈炎、46例不同程度宫颈不典型增生、15例原位癌和20例宫颈鳞癌组织中瘦素的表达情况,同时免疫细胞化学技术检测以上各病例宫颈脱落细胞中瘦素的表达。 |
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Methods The mice were immuned by polysaccharides of Angelica, Atractylodes and Giant typhonium rhizome respectively, then the corresponeding antibodies and cross-reactive antibodies in serum were detected by ELISA.
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方法取当归、白术、制白附子3种中药植物水溶性多糖为抗原,分别给予小白鼠免疫刺激,采用ELISA法检测小鼠血清中的相应抗体及交叉抗体。 |
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Immunohistochemical staining showed MMP-1 existed mainl.
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方法取正常大鼠心脏和通过钳夹大鼠心脏冠状动脉旋支制造缺血-再灌注模型的心肌组织,利用免疫组织化学、Westernblotting技术和计算机图像处理系统,对正常和缺血-再灌注大鼠心脏MMP-1的表达进行形态学观察,并对其含量进行定量分析。 |
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Methods The human breast cancer tissues and relatively normal breast tissues were collected from surgical operation and the samples of 21 patients with breast cancer were analyzed by immunocytochemistry.
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方法收集21例手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法对不同乳腺组织标本进行检测。 |
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Operation was performed after the infection was controled,pedicled cutaneous flap and myocutaneous flap were used to repair the defect.
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方法术前创口分泌物培养+药敏,清创,去除坏死组织和内固定,术中冲洗,术后敏感抗生素静滴抗炎,感染控制后择期应用带蒂皮瓣肌皮瓣修复创面并持续灌洗抗炎。 |
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OBJECTIVE To prepare brevescapine(BRE) inclusion complex freeze-dried powder injection and evaluate its safety.
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方法以2-羟丙基-β-环糊精(HP-β-CD)为包合材料,采用冷冻干燥法制备灯盏花素包合物冻干粉针,摩尔连续递变法确定包合物的包合比例,红外光谱法、差示扫描量热法对包合物进行验证,高效液相色谱法测定包合物前后水溶液中药物的溶解度,并考察灯盏花素冻干粉针剂的溶血性和血管刺激性。 |
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Objective To prepare tanshinone ⅡA inclusion complex freeze-dried powder injection and study its characters.
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方法以羟丙基-β-环糊精(HP--βCD)为包合材料,采用冷冻干燥法制备注射用丹参酮ⅡA包合物;用差示扫描量热法、红外光谱法及相溶解度法验证包合物的形成;考察注射用丹参酮ⅡA包合物复溶后的pH值、澄清度、渗透压、与稀释剂的配伍稳定性及溶血性等基本性质。 |
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Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out.
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方法应用RT-PCR、免疫印迹和免疫组织化学染色方法,检测出生后15d、35d、70d小鼠精囊腺、前列腺AQP1 mRNA、蛋白表达水平及细胞定位。 |
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Methods The inhibitory rate on cell proliferation of NSCLC in celecoxib group,DDP group,VP16 group,DDP with celecoxib group and VP16 with celecoxib group was detected by MTT assay.
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方法应用噻唑蓝比色法(MTT)检测顺铂(DDP)、足叶乙甙(VP16)单独应用及与塞来昔布联合应用的增殖抑制率,并根据金氏公式计算Q值,评价两药的合用效应。 |
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