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Methods:The objective gene to be mutated was cloned into pAlter Ex plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.
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方法 :将待突变的目的基因克隆到 pALTER Ex载体上 ,采用寡核苷酸介导的定点突变进行突变。 |
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Methods:The patients were treated with oxaliplatin 8mg/m~ iv infusion for h d combined with folinic acid 00mg iv infusion for h followed by -fluororacil 00mg/m~ continuos-infusion for h every weeks.
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方法:采用如下方案治疗晚大肠癌:奥沙利铂8mg/mivgtthd,亚叶酸钙00mgivgtthd,氟尿嘧啶00mg/mcivh,每周重复。 |
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Methods:The rationality of with Logistic regression was analyzed with logistic regression,and the influences factors of reform trend were sought for.
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方法:运用Logistic回归分析方法对基层卫生体制的合理性进行分析,从深层次挖掘出影响不同人群改革意向的因素。 |
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Methods:The serum levels of CA9 9 and CEA were determined in 8 cases with gastro intestinal cancer by enzyme immunometric assay.
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方法:采用酶联免疫吸附法对8例消化道恶性肿瘤患者的CA9-9、CEA水平进行测定。 |
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Methods:The stapler was used to resect the rectal lesion which was cm from anal.
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方法 :应用吻合器对 例距肛缘 ～ cm直肠占位性病灶及病变经肛进行了切除。 |
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Methods:The transnasal endoscope was applied in sinus surgery in cases,examination of the concealed portio in the ear,nose,and throat in 7 cases and biopsy in 0 cases.
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方法 :将鼻窦内窥镜用于鼻腔鼻窦手术 例 ,检查耳鼻咽喉隐匿区 7例 ,在鼻窦内窥镜指引下取组织活检 0例。 |
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Methods:To compare Tennison 's method(to repair cases)with Millard's( 7cases)in clinic.
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方法 :用三角瓣法修补单侧唇裂 例 ; 用旋转推进法修补 7例 ,并进行临床比较。 |
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Methods:To determinate the activity of serum CK in normal gravidas, placenta accreta and placenta increta gravidas respectively.
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方法:对 例正常产妇, 例胎盘粘连和例胎盘植入产妇分别测定其血清CK活性。 |
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Methods:To observe the effects of Qinyiheji supersonic inhaler on ovalbumin asthma guiea pig model with bronchalveolar avage fluid(BALF) E_(0) 、IL- 、IL-、GM-CSF、SIL-R、NO as index.
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方法:观察该方对卵白蛋白致敏哮喘动物模型支气管肺泡灌洗液(BALF)中Eo、IL- 、IL-、GM-CSF、SIL-R、NO的影响。 |
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Methods:To regulate and analyse about the achie vements in the study of Canavan recen tly.
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方法对近年Cana-van研究成果加以整理分析。 |
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Methods:To test 00 old people in comparison with the 00 members in healthy group by using urinate sugar test paper to test urinate sugar and using glucose oxydase to test blood sugar and blood sugar h after meal.
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方法:对00例老年人与健康对照组0例用尿糖测定试纸测定尿糖,用葡萄糖氧化酶法测定血糖及餐后h血糖。 |
