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Methods: Primacy cultured endothelial cells were treated with TNF-α(0 ng/ml) and angiotensin Ⅱ(μmol/L) in the absence and presence of PDTC( μmol/L).
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方法:用浓度为0 ng/ml TNF-α和浓度为μmol/L血管紧张素Ⅱ分别干预内皮细胞,TUNEL法检测细胞的凋亡情况、EMSA检测NF-κB的活性和Western-blot检测IκBα的表达; |
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Methods: Proceed general anesthesia by.
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方法 :应用麻醉时间短、迅速清醒的得普利麻 (Diprivan) ,门诊行纤维支气管镜检查及治疗。 |
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Methods: Rat model of cerebullar ischemia-and-reperfusion was made by using bulldog clamp incarcerating common carotid artery.
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方法:采用动脉夹箝闭双侧颈总动脉的方法,制作脑缺血再灌注大鼠模型。 |
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Methods: Rumination Scale,Empathy Scale, Tendency to Forgive Scale and Revenge Motivation Scale were administered to 9 college students.
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方法:采用沉思量表、移情量表、报复动机量表和宽恕倾向量表对9名大二学生进行问卷调查。 |
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Methods: Sixty patients scheduled for spinal anesthesia were randomly allocated to four groups with each. Group Ⅰ and Ⅱ were adult patients aged from to years old and group Ⅲ and Ⅳ were geratic aged patients of 7 years old.
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方法:0例ASAⅠ~ Ⅱ级择期手术病人根据年龄和是否预防性注射麻黄碱而分成四组,Ⅰ、Ⅱ组为~岁成年病人,Ⅲ、Ⅳ组为~7岁老年病人。 |
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Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega.
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方法: 采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究; |
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Methods: The ml of sodium hyaluronate, - Omg of triamcinolone acetonide and ml of % lidocaine were injected into kneecap of 0 patients every week for times.
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方法:0例 膝OA患者关节腔内注入%利多卡困ml和醋酸曲安奈德mg-0mg及透明质酸钠ml,每周一次,连续次为一疗程。 |
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Methods: The MRI materials from patients with congenital recessive spinal bifida (all of them were confirmed by operation and pathologic examination from 99 to 00) were analyzed.
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方法 :搜集 99~ 0 0 年经手术病理证实的 例儿童隐性脊柱裂MRI资料 ,分析其MRI表现。 |
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Methods: The active GGt in urine was measured by a continuous monitoring with γ glutamyle carboxy nirroanilide (GCNA) as substrate, which was recommended by International Federation of Chemistry Committee (IFFC).
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方法 :采用国际化学联合会(IFCC)推荐的以L 谷氨酸 羟基 对硝基苯胺 (γ glutamyle carboxy nirroanilide ,GCNA)为底物的连续监测法测定尿GGT活性。 |
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Methods: The anatomic features of suborbicularis oculus fat and orbital fat were observed on antiseptic cadaveric specimens and cases suffering from fullness and heaviness and ptosis of the lateral upper eyelid, who asked for blepharoplasty.
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方法:选用防腐固定标本例( 0侧),并对例(侧)上睑外侧臃肿肥厚下垂病例行上睑成形术,观察上睑眼轮匝肌下脂肪的位置、范围、厚度,与眶隔脂肪的关系。 |
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Methods: The invasiveness w as assessed by penetration through a m atrigel-coated filter, the proliferation was determ ined by the m ethod reported by Gillies and cellsurface ICAM- and sICAM- analyzed by flow cytom etry and ELISA.
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方法:根据细胞穿过铺有Matrigel胶多孔膜数量检测细胞侵袭力,用改良龙胆紫吸收比色计数法检测细胞增殖,以流式细胞分析和酶联免疫吸附试验检测ICAM-、sICAM-表达水平。 |