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The enzyme linked immunosorbent assay(ELISA) was used to measure the levels of tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),monocyte chemoattractant protein-1(MCP-1) and IL-6 in the serum,lung and renal cortex tissue homogenate in various groups.
中文意思:
用酶联免疫吸附法(ELISA)测定血清、肺和肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)及IL-6水平。


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The end cell density OD600 of the engineering strain pNSR-16/BL21(DE3)pLysS was 60-90, and the expression of the recombinant spider silk protein was up to the 11-12% of the total protein by the Do-stat fed-batch culture. 采用Do-stat补料分批发酵,最终菌体密度OD_(600)可在60-90范围,产物表达量约占总蛋白量的11~12%。
The enzyme activities of trichlorobenzene dioxygenase, dihydrodiol dehydrogenase, and catechol 1,2-dioxygenase, the enzymes were inspected and surveyed respectively. 在转化子中分别检测了三氯苯双加氧酶、脱氢酶、邻苯二酚1,2-双加氧酶的酶活性.
The enzyme activity reached 5.47U/mg crude protein when K. pneumoniae F-1 was induced for 4h by 0.5mmol/L IPTG. IPTG诱导表达研究表明,在IPTG浓度为0.5mmol/L时,诱导4h后甲酸脱氢酶表达明显; 发酵过程中甲酸脱氢酶比酶活达到5.47U/mg;
The enzyme digestion results showed that the six DNA samples could be digested by EcoRⅠ,and were suitable for further molecular biological experiments. 酶切实验结果表明,6种海洋微藻的基因组DNA都能被EcoRⅠ酶切,酶切图谱呈弥散状,满足分子水平操作的要求,可直接应用于进一步的分子生物学实验。
The enzyme extraction obtained with sterile water at 28 ℃ for 3h was used for the activity determination. 30℃固体培养70h,发酵后用无菌水(pH5.0)28℃浸提3h测定酶活性。
The enzyme linked immunosorbent assay(ELISA) was used to measure the levels of tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),monocyte chemoattractant protein-1(MCP-1) and IL-6 in the serum,lung and renal cortex tissue homogenate in various groups. 用酶联免疫吸附法(ELISA)测定血清、肺和肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)及IL-6水平。
The establishment of a method for the detection of the erythrocyte chemokine recepter and its clinical application 红细胞趋化因子受体测定方法的建立及临床初步应用
The establishment of animal models of recurrent aphthous ulcer with immunology 免疫方法建立复发性口腔溃疡动物模型
The establishment of the gene expression profile and cloning the genes associated with differentiation inducing of human glioma cells.* 人脑胶质瘤细胞诱导分化基因谱的建立及相关基因的克隆
The evaluation of radionuclide liver scanning for the diagnosis of 110 cases with primary hepatic carcinoma 肝扫描对原发性肝癌诊断的价值(附110例病例分析)
The experimental data from the following conditions: pressure: 70MPa, cell mass: water volume=l:10 suspended in deionized water with 2 cycles. 从而选择采用高压匀浆法批式循环,经2次破碎,操作压力为70MPa、菌体悬浮液浓度为:菌体质量:水体积=1:10。
 
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