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Total DNA extraction from Pericarpium Citri Reticulatae
中文意思:
陈皮总DNA提取方法的研究


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To explore clinical features and therapeutic evaluation of childhood anaplastic large cell non-Hodgkin's lym-phoma (CALCL), all clinical data were summarized and analyzed in 4 children with CALCL. 为探讨儿童间变型大细胞非霍奇金淋巴瘤(CALCL)临床特点、方案选择及疗效分析,对4例儿童间变型大细胞性非霍奇金淋巴瘤患儿进行临床诊断、免疫分型、治疗、随访及疗效总结。
To explore whether SEMA6A, B56, IGSF4 and SLIT2 is genes related to leukemia with promoter methylation ,the expression patterns of the 4 genes were detected with RT-PCR in HL60, U937 and MoM cell. 为探讨SEMA6A、B56、IGSF4和SLIT2在人类白血病是否存在甲基化失活,本研究应用RT-PCR检测了这4个基因在HL60、U937和Molt4等3种常见的白血病细胞系的表达情况;
To study T cells' recognition, activation and frequency distribution is the most important component element in modern immunology. T细胞是机体免疫系统最关键的细胞之一,研究T细胞的识别、活化及频数分布是现代免疫学基础研究及应用研究的一个最重要的环节。
Together with the advanced analysis method of LC/MS/MS, automatic sampling technique can dramatically improve the quality of pre-clinical research to meet the demand of high throughput drug screening. 若能把自动生物样品采集技术和现代先进的LC/MS/MS分析方法合并使用,则可显著提高临床前研究的速度和质量,以满足高通量药物筛选的要求。
Total DNA Extraction from Four Limestone Plants 4种石山植物DNA提取方法筛选
Total DNA extraction from Pericarpium Citri Reticulatae 陈皮总DNA提取方法的研究
Total Hepatic Irradiation by Cobalt - 60 Moving Strip Tech nique Short Term Result of 14 Cases of Advanced Primary Liver Cancer ~(60)钴移动条技术全肝照射晚期原发性肝癌14例报告
Total Iron and Protein Contents of Six Sorts of Animal Blood 六种动物血的铁和蛋白质含量
Total RNA was extracted from the human Molt-4 cells as template according to the instructions and then human CD81 gene was was amplified by RT-PCR and PCR. The PCR products was determined by Agarose gel electrophoresis. 根据已公布的人CDS IcDNA序列(核酸库中的编号:NM-004356),设计一对引物,然后取传代后的人Molt一4细胞,按照产品操作说明书从人Molt一4细胞提取总RNA,运用RT一PcR及PcR方法扩增出人CD81基因,PCR产物经l%琼脂糖凝胶电泳鉴定大小正确。
Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%. 以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3%。
Totally 128 differential expressed genes might be related to azoospermia were found by the cDNA microarray hybridization, among them 56 were up regulated genes and 72 were down regulated. 2、共获得128个可能与无精子症相关的差异表达基因,其中56个基因表达上调,72个基因表达下调,cDNA微矩阵杂交结果经原位杂交证实。
 
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