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Methods CD117~+ hemopoietic stem cells (HSCs) were isolated and purified from the bone marrow of healthy C57 mice by magnetic affinity cell sorting.
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方法取健康C57小鼠骨髓,通过MACS系统分离、纯化CD117~+造血干细胞; |
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Methods Collecting 106 698 data from Medical Examination Center of Shenzhen People's Hospital from 2003 to 2005,and analyzing liver function,blood fat,blood sugar,Hepsati-B antigon,tumor index according to different age groups.
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方法:收集2003年至2005年在深圳市人民医院体检中心体检人员的资料106698例,按年龄分组对肝功能、血脂、血糖、乙型肝炎表面抗原(HBsAg)、肿瘤标志物的实验室结果进行分析。 |
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Methods Comparative genomic hybridization(CGH)was used in screening DNA copy number changes along all chromosomes in 23 cases of primary gastric cancer.
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方法采用比较基因组杂交分析23例原发性胃癌基因组DNA拷贝数变化情况。 |
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Methods Detect the levels of VEGF in the tissue and serum by immunohistochemical staining and enzyme-linked immunosorbent assay(ELISA)in 112 patients with NSCLC,and analysed their clinical significances.
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方法应用免疫组织化学方法和酶联免疫吸附试验检测112例NSCLC患者的肺癌组织和血清中VEGF的表达,并分析其临床意义。 |
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Methods Experimental ulcerative colitis rats were made by the method of immunology and their immunological function and SOD in serums were oberved.
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方法 用免疫法制成大鼠溃疡性结肠炎实验模型 ,测定其血清有关免疫指标和SOD含量等 ,并与正常大鼠对照。 |
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Methods Front wall midmyocardial of left ventricular were isolated from New Zealand white rabbits . ICa-L in 10-6,10-5mol/L ibutilide was recorded using whole cell patch clamp techniques.
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方法用全细胞膜片钳技术记录10-6,10-5mol/L伊布利特细胞外液对兔正常左室中层心肌细胞L型钙通道电流(ICa-L)活性的影响。 |
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Methods Gelatin, collagen and RGD peptide Arginine Glycine Aspartic acid were physically adsorbed on PS surfaces, XPS was employed to investigate the variation of nitrogen content on the modified surfaces to validate the existence of proteins.
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方法采用物理包被法依靠疏水作用在PS表面架构明胶、胶原和RGD(精氨酸-甘氨酸-天冬氨酸)多肽的生物活性层。 通过光电子能谱(XPS)分析修饰表面的元素含量变化,N元素含量显著提高,说明蛋白分子在表面存在。 |
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Methods HBV DNA S gene were amplified by polymerase chain reaction(PCR) in sera from 36 children with HBsAg positive after immunized with hepatitis B vaccine. The PCR products were sequenced.
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方法收集乙肝疫苗免疫后HBsAg阳性的儿童血清标本36份,利用聚合酶链反应(PCR)扩增HBV S基因片段,对PCR产物进行脱氧核糖核酸(DNA)序列测定。 |
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Methods Levels of Lp(a) were measured with enzyme-linked immunosorbent assay(ELISA) and genetic phenotypes of Apo(a) were mapped using SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting analysis.
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方法应用酶联免疫吸附试验测量血清脂蛋白(a)浓度应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western blotting技术显示载脂蛋白遗传表型。 |
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Methods Neural stem cells both from prime culture(P0) and twice-passaged culture(P2) were tested by enzyme immunoassay for proliferation and fluorescent activated cell sorting for apoptosis by Annexin V-FITC and propidium iodide.
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方法原代培养后(P0)和经过两次传代后(P2)的神经干细胞用BrdU细胞增生检测试剂盒检测增生能力; 用Annexin V-FITC凋亡试剂盒检测早期凋亡率; |
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Methods Plasma levels of oxLDL and sICAM-1 were measured by enzyme-linked immunosorbent assay(ELISA) in 50 women with PE, including 25 mild preeclampsia(MPE) and 25 severe preeclampsia(SPE), and 25 normal pregnant women.
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方法采用酶联免疫吸附法(enzyme-linked immunosorbent as-say,ELISA),检测50例PE患者[PE组,其中子癎前期轻度(mild preeclampsea,MPE)及子癎前期重度(severe preeclampsia,SPE)各25例]及25例同期正常孕妇(对照组)血浆中oxLDL和sICAM-1的水平变化。 |
