|
Isolation, Expression and Function Analysis of Stress-related Genes in Plants
|
|
|
植物逆境相关基因的克隆、表达及功能分析 |
|
Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kütz in Dianchi Lake
|
|
|
滇池铜绿微囊藻(M.aeruginosa Ktz)的分离培养与总DNA提取的改进 |
|
Isolation, culture and identification of human pancreatic stem cells and their differentiation characteristics in vitro
|
|
|
人胰腺干细胞的体外分离培养、鉴定和分化特性 |
|
Isolation,cultivation and identification of chicken embryonic stem cells
|
|
|
鸡胚胎干细胞的分离、培养和鉴定 |
|
Isolation,culture and identification of murine embryonic stem cells of Kunming species
|
|
|
昆明种系小鼠胚胎干细胞的分离培养及特性鉴定 |
|
Isolation、Identification and Sequence Analysis of Differential Expressed Genes Induced by IL-1β in NIT-1 Pancreatic β-Cells
|
|
|
IL-1β诱导后NIT-1胰岛β细胞差异表达基因的分离、监定和序列分析 |
|
It indicate that the insecticidal protein in the supernatant is thermal instable, which corresponding with the property of Vip3A protein.
|
|
|
这说明上清蛋白含有一种对夜蛾科有高毒力和热不稳定性的蛋白,这与Vip3A蛋白的性质一致,从而确立了这种检测和鉴定方法的可行性。 |
|
It is resulted that the percentage of supercoiled pUDKH in plasmid is more than 95%,and RNA undetected. The content of host protein remained is 0.0166 u g/mg and chDNA is less than 2ng/mg,endotoxin is 0.00178EU/mg.
|
|
|
结果表明:质粒中超螺旋pUDKH含量达95%(质量百分比)以上,残留核糖核酸RNA检测不到,宿主蛋白含量小于10mg/mg质粒DNA,残留宿主DNA含量小于2ng/mg质粒DNA,内毒素含量约0.00178EU/mg。 |
|
It is showed that the cell parameters increased with Zr~(4+)increasing and KTP octahedron crystal was badly distorted.
|
|
|
随着掺杂Zr~(4+)浓度的增加,晶胞参数明显增大,KTP中八面体畸变的程度会更高。 |
|
It means that the two genes, ETV5 and DKFZ, may be involved in the cell proliferation.
|
|
|
推测ETV5、DKFZ两个基因可能与细胞的增殖相关。 |
|
It showed BL21(DE3) was the better bacterial host. The method of pH-stat fed-batch culture was applied to obtain high cell density. The final cell density OD600 of grown pNS2/BL21(DE3) was 71.64 and the volumetric productivity was 0.8g/L.
|
|
|
研究了重组质粒pNS2在两种宿主菌中的摇瓶与10L发酵罐培养表达情况,确定了较适的宿主菌为BL21(DE3),采用pH-stat补料分批发酵,工程菌pNS2/BL21(DE3)最终菌体密度OD_(600)达71.64,表达量为0.8g/L。 |