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coli BL21(pGCPI) cell growth and expression in the LB medium in shake flask were optimized.
中文意思:
coli BL21(pGCPI)可合成约占菌体总蛋白22.6%的可溶性reCPI-GST融合蛋白。


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coli ,the expressing product was 31.5% of the total cell protein, its enzyme activity was 38.5 U/mg. 表达产物占菌体总蛋白的31.5%,酶活为38.5 U/mg.
coli BL21(pGCPI) cell growth and expression in the LB medium in shake flask were optimized. coli BL21(pGCPI)可合成约占菌体总蛋白22.6%的可溶性reCPI-GST融合蛋白。
coli HB101 (pMB4) strain. The activity of this enzyme was determined. 从大肠杆菌HB101(PMB_4)中制备出高活性的EcoRI酶,并鉴定其生物活性。
continuously feeding H2O2 solution to the broth was started as the cell’s incubation 96 h upto its final content 5 mmol/L, and the conversion rate was kept at a high level. 菌体培养96 h始流加H2O2,最终浓度达到5 mmol/L能够大幅度提高转化率。
de novo Design and Conformational Studies of a β-hairpin Forming Peptide β-发夹多肽的全新设计和构象研究(英文)
dehydroquinate dehydratase was a(α2/β2)3 peptide,of which a pair of anti-parallel β-strands existed in the N-terminus and a loop in the C-terminus; 脱氢奎尼酸酶结构域为(α2β2)3多肽,在N-端有一对反平行的β链,在C-端有loop环;
experimental group that uses the factors which influence the proliferation and differentiation of neural stem cells. 实验组为采用干扰神经干细胞增殖与分化的因素。
 
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