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Immunohistochemical method was used to observe the expression and distribution of HSP 70 in the rats retina.
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方法 SD大鼠在高温环境生存 2h ,分别于 4、18、2 4、48h后摘除眼球 ,用免疫组织化学方法观察大鼠视网膜中HSP 70的表达和分布。 |
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Methods 200 cases of breast cancers and 200 cases of benign breast disease were divided into two groups using paired design.
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方法 对 2 0 0例乳腺癌和 2 0 0例良性乳腺疾病患者用生活事件调查表进行调查。 |
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Methods DHPLC and DNA sequencing were used to examine the amyloid precursor protein and presenilin 1(PS1) gene of a 130 member family and a 50 member control group.
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方法 应用 DHPLC方法及 DNA直接测序技术 ,对一个有 1 3 0人的AD大家系和 5 0名正常对照者进行了淀粉样蛋白前体 (amyloid precursor protein,APP)基因、早老素 1 (prese-nilin1 ,PS1 )基因的突变位点检测。 |
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Methods: The immunohistochemical method was used to examine the expressions of CD 44V 6, MMP-9 and CATH-D protein in 128 cases of stomach carcinoma.
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方法 应用免疫组织化学技术检测CD44V6、MMP 9和CATH D蛋白在 12 8例胃癌中的表达。 |
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Methods Serum samples were tested for anti-SLA/LP by indirect immunofluorescence assay (IIF) and Western blot(WB), and the positive rate was compared with those of anit-SMA, anti-AMA-M2 and anti-LKM-1.
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方法 用间接免疫荧光法、免疫印迹法检测抗SLA/LP抗体,并与抗平滑肌抗体(SMA)、抗线粒体抗体(AMA M2)及抗肝肾微粒体(LKM 1)抗体之间的阳性率进行比较。 |
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hSel P137m was split by hydroxylamine and the result was analyzed by SDS-PAGE and Western blotting.
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方法: 利用生物信息学技术分析hSelP280m,hSelP137m的活性功能域,用羟胺切割hSelP137m,并用SDSPAGE及蛋白质印迹加以分析验证。 |
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Methods According to mastic preparation craft mastic of zinc norfloxacin was made and some methods like the character,the distinction and the potential of hydrogen were put forward and external inhibition bacterium experiment was performed.
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方法:按凝胶剂制备工艺制备了诺氟沙星锌凝胶剂;建立了性状、鉴别、酸碱度、含量测定等质控方法,并进行了体外抑菌实验。 |
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Objective:To investigate the mechanism of Dicha Kechuan Oral Liquid in treatment of chronic bronchitis model mice.
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方法:采用改进烟熏方法复制慢性支气管炎小鼠动物模型,将小鼠随机分为6组:模型组、复方川贝母组、地茶咳喘露高、中、低组及不加处理的正常组。 |
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Objective: To study the expressions and clinical significances of p16INK4A and p14ARF proteins in squamous carcinoma of the cervix(SCC) and cervical intraepithelial neoplasia(CIN).
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方法:采用免疫组织化学S-P法对95例宫颈鳞癌(SCC)标本、80例宫颈上皮内瘤变(CIN)标本和45例正常宫颈标本进行p16INK4A和p14ARF蛋白的检测。 |
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Methods: The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.
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方法:采用全细胞膜片钳电生理方法,记录氧化还原药物对三叉神经节细胞大电导钙激活钾通道(BKCa)的影响。 |