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The effects of iodine and selenium deficiency on permeability of thyroid cell in rats
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碘硒缺乏对大鼠甲状腺细胞膜通透性影响——碘硒对甲状腺影响研究之Ⅲ |
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The effects of reaction conditions, such as substrate concentration, inhibition of product, reaction pH and temperature, co-substrate and cell culture condition, on the reaction were investigated.
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同时发现在水相体系中反应,存在一定程度的副反应。 实验考察了底物浓度、产物的抑制、反应体系的pH、反应温度以及辅助底物等反应条件和菌体培养条件对酵母细胞不对称还原COBE的影响。 |
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The eight cDNAs from C/SH/F/98 were cloned into the bidirectional transcription/expression vector pHW2000, and a panel of reasserted viruses containing HA and NA genes from C/SH/F/98 were generated by reverse genetics technique.
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8个基因的序列已发表在GenBallk，登录号为AY250750一AY250756，AF46 1 532。 C/SH/F/98成为在GenBank中第一个登录序列完整的HgNZ亚型流感病毒株，也是中国大陆第一个自主登录基因组全长序列的HgNZ亚型毒株。 |
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The end cell density OD600 of the engineering strain pNSR-16/BL21(DE3)pLysS was 60-90, and the expression of the recombinant spider silk protein was up to the 11-12% of the total protein by the Do-stat fed-batch culture.
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采用Do-stat补料分批发酵,最终菌体密度OD_(600)可在60-90范围,产物表达量约占总蛋白量的11～12%。 |
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The enzyme activities of trichlorobenzene dioxygenase, dihydrodiol dehydrogenase, and catechol 1,2-dioxygenase, the enzymes were inspected and surveyed respectively.
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在转化子中分别检测了三氯苯双加氧酶、脱氢酶、邻苯二酚1,2-双加氧酶的酶活性. |
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The enzyme activity reached 5.47U/mg crude protein when K. pneumoniae F-1 was induced for 4h by 0.5mmol/L IPTG.
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IPTG诱导表达研究表明,在IPTG浓度为0.5mmol/L时,诱导4h后甲酸脱氢酶表达明显; 发酵过程中甲酸脱氢酶比酶活达到5.47U/mg; |
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The enzyme digestion results showed that the six DNA samples could be digested by EcoRⅠ,and were suitable for further molecular biological experiments.
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酶切实验结果表明,6种海洋微藻的基因组DNA都能被EcoRⅠ酶切,酶切图谱呈弥散状,满足分子水平操作的要求,可直接应用于进一步的分子生物学实验。 |
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The enzyme extraction obtained with sterile water at 28 ℃ for 3h was used for the activity determination.
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30℃固体培养70h,发酵后用无菌水(pH5.0)28℃浸提3h测定酶活性。 |
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The enzyme linked immunosorbent assay(ELISA) was used to measure the levels of tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),monocyte chemoattractant protein-1(MCP-1) and IL-6 in the serum,lung and renal cortex tissue homogenate in various groups.
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用酶联免疫吸附法(ELISA)测定血清、肺和肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)及IL-6水平。 |
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The establishment of a method for the detection of the erythrocyte chemokine recepter and its clinical application
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红细胞趋化因子受体测定方法的建立及临床初步应用 |
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The establishment of animal models of recurrent aphthous ulcer with immunology
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免疫方法建立复发性口腔溃疡动物模型 |
