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cDNA Library Construction of Wheat Near Isogenic Line with Disease Resistance to Powdery Mildew and Full-length cDNA Cloning of the Genes Related to Disease Resistance
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小麦抗白粉病近等基因系cDNA文库构建及抗病相关基因的全长cDNA克隆 |
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coli ,the expressing product was 31.5% of the total cell protein, its enzyme activity was 38.5 U/mg.
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表达产物占菌体总蛋白的31.5%,酶活为38.5 U/mg. |
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coli BL21(pGCPI) cell growth and expression in the LB medium in shake flask were optimized.
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coli BL21(pGCPI)可合成约占菌体总蛋白22.6%的可溶性reCPI-GST融合蛋白。 |
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coli HB101 (pMB4) strain. The activity of this enzyme was determined.
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从大肠杆菌HB101(PMB_4)中制备出高活性的EcoRI酶,并鉴定其生物活性。 |
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continuously feeding H2O2 solution to the broth was started as the cell’s incubation 96 h upto its final content 5 mmol/L, and the conversion rate was kept at a high level.
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菌体培养96 h始流加H2O2,最终浓度达到5 mmol/L能够大幅度提高转化率。 |
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de novo Design and Conformational Studies of a β-hairpin Forming Peptide
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β-发夹多肽的全新设计和构象研究(英文) |
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dehydroquinate dehydratase was a(α2/β2)3 peptide,of which a pair of anti-parallel β-strands existed in the N-terminus and a loop in the C-terminus;
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脱氢奎尼酸酶结构域为(α2β2)3多肽,在N-端有一对反平行的β链,在C-端有loop环; |
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experimental group that uses the factors which influence the proliferation and differentiation of neural stem cells.
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实验组为采用干扰神经干细胞增殖与分化的因素。 |
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four patients were screened for expression of 4366 human genes, leading to the identification of 4 genes with elevated expression and 11 genes with reduced expression in the glioma tissues.
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用含有4366个基因的芯片对4例胶质瘤标本与正常脑进行了差异表达谱分析，发现4个基因在胶质瘤中表达上调，11个基因在胶质瘤中表达下调。 |
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hMSH2 deficiency of DNA repair enzyme and cell genetic stability
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DNA修复酶hMSH2缺陷与细胞遗传不稳定性 |
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in vivo and in vitro Anti-Tumor Efficiency by Tumor-Specific CD8+ CTL Induced with Tumor Associated Peptide
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肿瘤抗原多肽诱导的CD8~+CTL的体内外抗瘤效应 |
